Protocols

1. Agarose gels of DNA
2. Electrocompetent E.coli cells
3. Bacterial electroporation
4. Preparation of media
5. Preparation of agar plates
6. Electroporation of libraries
7. SDS-PAGE on vertical mini gels
8. Western blotting
9. Preparation of affinity resins
10. Protein purification using the NiNTA resin
11. Preparation of phage particles from phage vectors
12. PCR
13. PCR screening
14. Gene shuffling
15. Running 2D-PAGE
16. Silver staining of 2D-gels
17. Coomassie staining of 2D-gels
18. Scanning 2D gels
19. In-gel digestion of proteins
20. SYPRO Ruby Protein Gel Stain
21. Affinity measurements by band-shift assay
22. Affinity measurements by competition ELISA
23. Protein expression in Pichia
24. Culturing HEK 293 Cells
25. Culturing fibroblast
26. Culturing endothelial cells
27. Transfection of kidney cell line.
28. Generating stable cell lines in HEK293
29. Protocols for the preparation of tumour cells for s.c. injections
30. Preparation of cryopreserving medium
31. Protocols for the preparation of cryotubes of tumour cells
32. Protocols for the CAM assay
33. Protocols for the immunohistochemistry
34. Pellet preparation for the cornea micropocket assay
35. DNA sequencing using the ABI Prism sequencer
36. ETH-2 library protocols: on-line or in Word format
37. Buffers
38. Praktikum1
39. Praktikum2