BUFFERS

TBS (Tris buffered saline)
50 mM Tris
100 mM NaCl

PBS (Phosphate buffered saline)
20 mM NaH2PO4 (27,6 g with MW 138 and in 10 l water)
30 mM Na2HPO4 (107,4 g; MW 358)
100 mM NaCl (58,4 g; MW 58.4)

10 x TBE
108 g/l Tris
55 g/l Borsäure
8 ml 0,5 M EDTA pH8,0

TE
10 mM Tris
1 mM EDTA

6 x loading buffer (DNA)
4 g saccharose
25 mg  bromophenol blue
water ad 10 ml

3 x loading buffer (protein)
25 ml 0,5 M Tris pH6,8
20 ml glycerol
40 ml10% SDS
40 mg bromophenol blue
14 ml water
just before use: add 10 µl 2-mercaptoethanol to 1 ml of buffer

5 x SDS-page running buffer
75.7 g Tris base
360 g glycine
125 ml 10 % SDS
water ad 2,5 l

100 mM triethylamine
696 µl triethylamine
ad 50 ml water

20 x dNTP
5 mM dATP
5 mM dCTP
5 mM dGTP
5 mM dTTP

LB
10 g bacto-tryptone
5 g  bacto-yeast-extract
10 g NaCl
water ad 1 l
add 200 µl 10 M NaOH
autoclave

2 x YT
16 g bacto-tryptone
10 g  bacto-yeast-extract
5 g NaCl
water ad 1 l
add 150 µl 10 M NaOH
autoclave