Coomassie staining
-
Wash
After the 2nd dimension was complete, gels were rinsed in water (MilliQ)
for 5 minutes.
-
Fixation
2D gels were fixed in a 50% (v/v) methanol, 10% (v/v) acetic acid solution
for at least 4 hours or over night (1-2 hours for minigels) It was made
sure that the gels were visibly shrinking.
-
Staining
Gels were stained in a 0.025% ( w/v) Coomassie Brilliant Blue G in
10% acetic acid solution for 3 hours (30-50 minutes for minigels). The
solution with the coomassie blue was stirred for at least 45 minutes before
use.
-
Destainig
Gels were destained in 10% acetic acid till the background was transparent.
Destain solution was replaced at least twice.
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