printlogo
http://www.ethz.ch/index_EN
Biomacromolecules
 
print
  

Culturing HEK 293 Cells

Reagents

Procedures

Starting from a Kryotube:
Prewarm the medium at 37°C and fill two culture flasks (25 ml for 150 cm2, 5 ml for 25 cm2). Rapidly thaw the cells (at 37°C) and distribute them in two concentrations in the flasks.
Change the medium after 12 hrs or once the cells have attached.


Splitting Cells:
Aspirate the medium from the flask. Wash the cells carefully with PBS to remove residual medium. Add 1-2ml of Trypsin Solution (equilibrated at RT) to the flask (150 cm2) and incubate at 37°C until cells have detached (1-2 minutes). Prepare a new flask with fresh medium. Block trypsinization by adding a few ml of medium. Take a fraction of the cell solution and inocculate the new flask. Typically, when splitting confluent HEK 293 cells in a 1:10 ratio, confluency is reached again after 2-3 days.

 

Wichtiger Hinweis:
Diese Website wird in älteren Versionen von Netscape ohne graphische Elemente dargestellt. Die Funktionalität der Website ist aber trotzdem gewährleistet. Wenn Sie diese Website regelmässig benutzen, empfehlen wir Ihnen, auf Ihrem Computer einen aktuellen Browser zu installieren. Weitere Informationen finden Sie auf
folgender Seite.

Important Note:
The content in this site is accessible to any browser or Internet device, however, some graphics will display correctly only in the newer versions of Netscape. To get the most out of our site we suggest you upgrade to a newer browser.
More information

© 2014 ETH Zurich | Imprint | Disclaimer | 13 December 2005
top