SYPRO, Ruby Protein Gel Stain

MB 18.9.00

After the 2nd Dimension, when the strip is removed:

1. Fix the gel in 10% Methanol and 7% acetic acid for 30 minutes.

2. Remove the fix solution and incubate the gel in the undiluted stain for at least 90 minutes. For convenience, gel may be left in the dye solution overnight or longer without overstaining.

Recommended amount of stain solution per gel:

Or 10 times the volume of the gel for other gel sizes.

3. Rinse the gel in deionized water for 30-60 minutes.

4. Optional: To decrease background fluorescence and to reduce speckling, the gel can be washed in a mixture of 10% methanol and 7% acetic acid.

5. The gel can be either dried for permanent storage or the spots can be cut and used for Edman sequencing or peptide mass fingerprinting.


Material needed:

Methanol gradient grade, HPLC (Unischaltercode: 123)
Acetic acid, Fluka, puriss. p.a., Code: 45731
SYPRO’ Ruby protein gel stain, (Molecular Probes, Code:S-1200)